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DIPP: Dual Isotope Paulina Polder
Citation
Veuger and Van Oevelen, 2156: Data from Dual Isotope labeling at Paulina Polder. etherlands Institute of Ecology; Centre for Estuarine and Marine Ecology, Netherlands. Metadata available at http://mda.nioo.knaw.nl/imis.php?module=dataset&dasid=2156. https://marineinfo.org/doc/dataset/2156

Archived data

Archived file:
Availability: Restricted
The data are withheld from general circulation and disclosure but access may be obtained on a case-by-case basis through negotiation

Description
Available data from the experiment in which sediment was injected with 13C-glucose and 15N-ammonium. Experiment was conducted 1) in situ, 2) sediment cores and 3) slurry bottles more

13C-glucose and 15N-ammonium was injected in three ways:
1) in situ sediments, 4 week incubation
2) retrieved sediment cores, 4 week incubation
3) slurry bottles, one year incubation
The different treatments were sampled for:
  • 13C/15N incorporation in POM
  • 13C incorporation in PLFA
  • 13C incorporation in FA
  • 13C incorporation in sugars
  • 13C/15N incorporation in Hydrolyzable amino acids
  • 13C/15N incorporation in meio- and macrofauna
  • pigment composition
  • three macrofaunal species were analyzed for 13C/15N in FA and HAAs


Scope
Themes:
Biology, Biology > Benthos, Biology > Invertebrates, Biology > Organic (& bio-) chemistry, Bottom samples (e.g. core, dredge, grab)
Keywords:
Marine/Coastal · Bacteria · Benthic infauna · Carbon isotopes · Macrobenthos · Meiobenthos · Nauplii · Nitrogen isotopes · Pigments · Radioactive labelling · Sediments · PaulinaSchor · Arenicola marina (Linnaeus, 1758) · Arenicola marina (Linnaeus, 1758) · Bacteria · Bivalvia · Brachyura · Cerastoderma edule (Linnaeus, 1758) · Copepoda · Eteone Savigny, 1822 · Foraminifera · Glycera Lamarck, 1818 · Heteromastus filiformis (Claparède, 1864) · Macoma balthica (Linnaeus, 1758) · Nematoda · Nematoda · Nereis diversicolor O.F. Müller, 1776 · Oligochaeta · Ostracoda · Polychaeta · Pygospio elegans Claparède, 1863 · Scrobicularia plana (da Costa, 1778) · Tharyx marioni (Saint-Joseph, 1894)

Geographical coverage
PaulinaSchor [Marine Regions]

Temporal coverage
14 June 2006 - 20 February 2007

Taxonomic coverage
Arenicola marina (Linnaeus, 1758) [WoRMS]
Arenicola marina (Linnaeus, 1758) [WoRMS]
Bacteria [WoRMS]
Bivalvia [WoRMS]
Brachyura [WoRMS]
Cerastoderma edule (Linnaeus, 1758) [WoRMS]
Copepoda [WoRMS]
Eteone Savigny, 1822 [WoRMS]
Foraminifera [WoRMS]
Glycera Lamarck, 1818 [WoRMS]
Heteromastus filiformis (Claparède, 1864) [WoRMS]
Macoma balthica (Linnaeus, 1758) [WoRMS]
Nematoda [WoRMS]
Nematoda [WoRMS]
Nereis diversicolor O.F. Müller, 1776 [WoRMS]
Oligochaeta [WoRMS]
Ostracoda [WoRMS]
Polychaeta [WoRMS]
Pygospio elegans Claparède, 1863 [WoRMS]
Scrobicularia plana (da Costa, 1778) [WoRMS]
Tharyx marioni (Saint-Joseph, 1894) [WoRMS]

Parameters
Amino acids Methodology
C:N ratio Methodology
Carotene Methodology
Chlorophyll a Methodology
Chlorophyll c Methodology
Diatoxanthin Methodology
Fucoxanthin Methodology
Isotope Carbon (C)
Isotope Nitrogen (N)
Organic carbon (OC) Methodology
Organic nitrogen (N) Methodology
Phaeophytin Methodology
Phospholipid-derived fatty acids (PLFAs) analysis Methodology
Pigment analysis Methodology
Amino acids: Amino acids in sediment measurements
As described by: Veuger, B. et al. (2005). Analysis of 15N incorporation into D-alanine: a new method for tracing nitrogen uptake by bacteria. Limnol. Oceanogr: Methods 3: 230-240.

Amino acids: Amino acids in sediment measurements
As described by: Veuger, B. et al. (2005). Analysis of 15N incorporation into D-alanine: a new method for tracing nitrogen uptake by bacteria. Limnol. Oceanogr: Methods 3: 230-240.
C:N ratio: EA-IRMS
Carotene: HPLC
Chlorophyll a: HPLC
Chlorophyll c: HPLC
Diatoxanthin: HPLC
Fucoxanthin: HPLC
Organic carbon (OC): EA-IRMS
Organic nitrogen (N): EA-IRMS
Phaeophytin: HPLC
Phospholipid-derived fatty acids (PLFAs) analysis: Phospholipid fatty acid analysis (PLFA) by GC-c-IRMS methodology
Methodology is based on: Boschker, H. T. S. 2004. Linking microbial community struc-ture and functioning: Stable isotope (13C) labeling in com-bination with PLFA analysis, p. 1673-1688. In G. A.Kowalchuk, d. B. F.J., I. M. Head, A. D. Akkermans, and J. D.van Elsas [eds.], Mol.

Phospholipid-derived fatty acids (PLFAs) analysis: Phospholipid fatty acid analysis (PLFA) methodology
Pigment analysis: HPLC

Contributor
Royal Netherlands Academy of Arts and Sciences; Netherlands Institute of Ecology (NIOO-KNAW), moredata owner

Dataset status: In Progress
Data type: Data
Data origin: Data collection
Metadatarecord created: 2009-10-21
Information last updated: 2013-09-10
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